Part:BBa_K2637033:Design

CikA cassette with TEF1 Promoter and TEF1 Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 644
  Illegal BamHI site found at 2704
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 266
  

Design Notes

TEF1 Promoter + CikA + TEF1 Terminator. And CikA has been optimized in yeast.

Source

TEF1P and TEF1T are the promoter and terminator of yeast. CikA is from Synechococcus elongatus PCC7942.